ABSTRACT
Dead and damaged spermatozoa cells present universally in the ejaculates of all eutherian mammals exert toxic effect on contemporary healthy cells mostly through generation of excessive free radicals. This is much more evident during extended period of processing, resulting in poor ejaculate quality. The solution lies in depletion of dead/damaged spermatozoa from the neat ejaculates itself. Thus the objective of the study was to evaluate the efficiency of the protocols such as discontinuous PercollTM density gradient centrifugation (PDGC) and glass wool filtration (GWF) for depletion of dead/damaged spermatozoa from fresh semen in buffalo. Random ejaculates (n=6) of Murrah buffalo bulls were divided into two aliquots after quality assessment: PDGC and GWF protocols (Group I and II, respectively). At the end of the purification protocol, efficiency of the protocols in depleting dead/damaged spermatozoa as reflected by certain quality parameters were evaluated. The mean efficiency (%) of purification protocols based on recovery of spermatozoa was 44.68 and 40.02% for PDGC and GWF, respectively. Moreover significantly (p<0.05) greater values for quality parameters was observed in the Group II (26.4+6.8 vs 68.8+4.4 for acrosome integrity (%); 12.68+6.6 vs 57.7+7.5 for functional plasma membrane integrity (%); 20.3+5.8 vs 80.75+6.7 for viability (%) in Group I and II, respectively). It was concluded that GWF is a better technique than PGDC to filter out dead/damaged spermatozoa from fresh semen with improvement in semen quality and can be a valuable tool in assisted reproductive technology
ABSTRACT
Sirakamba vatham is a clinical entity described by Sage Yugi in his treatise Yugi Vaithiya Chinthamani- 800, as one among the 80 types of Vatha diseases described in Siddha system of medicine. The study was aimed at in depth analysis of the clinical features mentioned under Sirakamba vatham of Siddha literature and to evolve standard Siddha diagnostic methods for management for Sirakamba vatham. This study was an observational, single center study with the sample size of 26, divided into Group I (control group) having normal individuals and group II (cases with Sirakkamba vatham). At the end of the study, it was concluded, that the symptoms of Sirakkamba vatham closely resembled the symptoms of Cerebro Vascular Accident especially of posterior circulation stroke.
ABSTRACT
The Siddha system of medicine is widely practiced in South India and consists of an enormous classical literature that emphasize on pathological basis of disease. Contrary to conventional pathological basis of diseases, the Siddha pathology is solely based on the humoral makeup of individuals and rests on the conceptual framework formed by 96 Thathuvams (philosophies). These concepts connect the physical and inert energies of human body facilitating its existence at subtle and gross levels. Sirakkambavatham is one among the 80 Vatha diseases mentioned in the Siddha literature Yugivaithiyasinthaamani. The present literature survey has been conducted to provide an updated integrative framework of information about the pathological concepts of Sirakkambavatham from Siddha literature and its parallel analysis with Cerebrovascular accidents (Stroke).Validating the traditional text in the limelight of modern literature unveils the traditional wisdom of ancient saints of South India and provides a better approach for disease diagnosis, prevention and its management.